The invention discloses hydrogel with multiple response function and a preparation method and application thereof; the preparation method of the hydrogel comprises the steps of adding hydrogel material, acryloyl chloride and triethylamine into a solvent, and reacting under ice bath to obtain modified hydrogel material; adding diselenide-diol, acryloyl chloride and triethylamine into a solvent, reacting under ice bath to obtain modified diselenide with C-C double bond; mixing the modified hydrogel material solution with the modified diselenide solution, adding a photo-polymerization initiator, and irradiating in ultraviolet at 20-40 DEG C to generate hydrogel having multiple response function. The hydrogel material is responsive to temperature, light and redox, quick gelling can be achieved by adjusting temperature, bulk concentration, photo-polymerization initiator concentration and a light source, the hydrogel is easy to form and has excellent mechanical properties and biocompatibility, and redox responsiveness of the hydrogel helps load different drug molecules and release them effectively.