The invention discloses an experimental device and an experimental method for simulating the wave disturbance of a lake, belonging to the field of water environment experiment. In order to solve the problem that the experimental device in the prior art has the disadvantages of non-uniform environment, complex assembly and complicated operation, the invention provides an experimental device and an experimental method for simulating the wave disturbance of a lake. The experimental device comprises an experimental table base. The periphery of the experimental table base is equipped with a support. The support is arranged around and above the experimental table base. A sample culture pool is arranged on the experimental table base. A disturbance device is arranged on the support, and passes through the support into the sample culture pool. Motors rotate circularly to make support rods do up-down linear periodic motion. Under the action of frequency modulators on the motors, the motors rotate at the same speed, and the left and right support rods do different linear motion. By converting mechanical energy into potential energy, the irregular three-dimensional wave of a lake can be simulated. A realistic simulation environment is achieved. The device is simple, and easy to operate.