The invention discloses a unidirectional radiation holographic antenna. The structure of the unidirectional radiation holographic antenna includes a feed source antenna and a holographic structure, wherein the holographic structure includes an upper level medium substrate, a lower layer medium substrate and a metal holographic pattern; metal patterns are respectively printed on the upper level medium substrate and the lower layer medium substrate; and the lower layer medium substrate moves by a quarter of wavelength, relative to the upper level medium substrate, in the horizontal direction. The unidirectional radiation holographic antenna utilizes medium substrates with the same planar dimension and different upper and lower thickness to form a holographic structure without using a complicated structure. Besides, the double layer holographic structure can use any one source antenna, so that the structural design of the source antenna is simplified. In the perpendicular direction, the distance between the upper level medium substrate and the lower layer medium substrate can be calculated according to the radiation direction of the target antenna. The unidirectional radiation holographic antenna can use any antenna as a source antenna, can realize unidirectional radiation in any direction, can improve the holographic antenna radiation gain and increase the range of the wave beam scanning angle, is simple and flexible, and can be suitable for various application occasions.