A fertilizer for tea tree plantation and a preparing method thereof are disclosed. The fertilizer is prepared from ammonium nitrate, potassium sulfate, urea, ferric sulfate, zinc sulfate, plant ash, straw powder, bacillus powder, photosynthetic bacterium powder, dried livestock manure, dried poultry dung, a rooting agent, a plant activating enzyme, slaked lime and fish pond sludge powder. According to the method, the raw materials are separately crushed by a crusher, then the crushed raw materials are mixed and stirred by a stirrer, a proper amount of water is added into the mixture, the mixture is fully stirred, then the bacillus powder, the photosynthetic bacterium powder, the rooting agent and the plant activating enzyme are added into the mixture, the mixture is granulated by a granulating machine after the mixture is fully stirred, and fertilizer granules are dried by a dryer and spread and kept in a shade. The fertilizer integrates organic matters, trace elements and a plurality of microbes, can effectively improve soil quality, can increase quality of tea leaves, can increase the yield of tea leaves, and can effectively overcome problems of insufficient nutrition and slow growth in tea tree plantation. The using range of the fertilizer is wide. The fertilizer is convenient to use.