The invention discloses a system for digitalizing X-ray markers in spinal injury measurement. The system comprises a visualized interaction module, a picture display execution module, a digital IO execution module, an X-ray photo processing execution module and a three-dimensional calculation execution module, wherein the visualized interaction module is used for calling a corresponding execution modules to execute corresponding operations according to control instructions, and displaying operation results which are fed back; the picture display execution module is used for taking corresponding X-ray photos and sending the X-ray photos to the visualized interaction module; the digital IO execution module is used for forming a configuration file according to a numerical value of each input predetermined parameter, and updating the configuration file by utilizing data which is fed back; the X-ray photo processing execution module is used for identifying a calibration bead and a terminal plate bead by utilizing the configuration file and calling an X-ray photo processing program, extracting a network cable, executing a surrounding acupuncture digitalization process, and feeding data obtained after the X-ray photo processing program is completed back to the digital IO execution module; and the three-dimensional calculation execution module is used for calculating three-dimensional coordinates of markers in X-ray photos by utilizing the configuration file and calling a three-dimensional coordinate calculation program. According to the system, all the digital work is integrated to a uniform interface so that convenience is provided for the X-ray digitalization.