The invention discloses a steroid compound and application thereof. The steroid compound is a compound having a structure shown as a formula I or pharmaceutically acceptable salt, tautomer, stereisomer, a precursor compound, hydrate or solvate of the compound (show in the description). An experiment shows that H on the steroid compound C10 having the structure shown as the formula I is substituted by a hydrogen peroxide group, the cardiac muscular tension of the frog heart can be remarkably increased, the steroid compound has a remarkable cardiotonic effect, the cytotoxicity is lower, and the steroid compound is efficient and low in toxicity, can be expected to serve as an active ingredient for preparation of drugs for preventing and/or treating acute heart failure and has a wide application prospect.