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发明公开
CN107058372A 一种应用于植物上的CRISPR/Cas9载体的构建方法 审中-实审

一种应用于植物上的CRISPR/Cas9载体的构建方法
摘要：
本发明涉及一种应用于植物上的CRISPR/Cas9载体的构建方法，其包括：S1：靶序列退火复性；S2：PSG载体的酶切；S3：连接和转化；S4：重组质粒的鉴定和提取；S5：重组资料和PCC质粒的双酶切；S6：连接、转化和鉴定。其获得的载体不仅能够作用于单个靶位点，而且能够同时作用于两个靶位点。
摘要（英）：

The invention relates to a method for constructing a CRISPR/Cas9 vector applied to a plant. The method comprises the following steps: S1: performing annealing renaturation on a target sequence; S2, performing enzyme digestion on a PSG vector; S3, connecting and transforming; S4, identifying and extracting a recombinant plasmid; S5, performing double enzyme digestion on recombination information and PCC plasmids; and S6, connecting, transforming and identifying. The obtained vector can act on a single target site and can also simultaneously act on two target sites.

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