The invention relates to a pressure power generation yarn and a preparation method thereof and belongs to the technical field of new energy and power generation. A conductive polymer fiber and aluminium-coated fiber blended yarn serves as a core yarn, the outer surface of the core yarn is covered with a conductive polymer fiber, and the core yarn of which the outer surface is covered with the conductive polymer fiber, and a metallized fiber yarn are parallelly intertwined into a whole to obtain the pressure power generation yarn. The prepared pressure power generation yarn has the advantages of simple process, excellent conductivity and flexibility, very good anti-static function and the like, can be widely applied to production of textiles with the piezoelectric conversion function, and has a wide application prospect in the fields of smart clothing, wearable displays, embedded health detection and nursing and the like.