The invention relates to an optical fiber dissolved oxygen sensor probe and a manufacturing method thereof, and aims at solving the problems that at present, the process of measuring oxygen through metalloporphyrin phosphorescence is complex, and real-time detection cannot be conducted. The optical fiber dissolved oxygen sensor probe comprises optical fiber and an oxygen sensing film, and the oxygen sensing film is cured on the optical fiber emitting end face through a coordination reaction; the oxygen sensing film is metalloporphyrin embedded cage type polysilsesquioxane. Accordingly, metalloporphyrin is cured to the optical fiber end face through the cage type polysilsesquioxane, the optical fiber probe can be utilized for directly detecting the dissolved oxygen concentration of a to-be-detected solution, the sampling step necessary for an ordinary detection method is not needed, and the measuring efficiency is improved; in addition, the metalloporphyrin has high response to dissolved oxygen, and real-time monitoring on the dissolved oxygen concentration can be achieved by means of the optical fiber probe. The optical fiber dissolved oxygen sensor probe and the manufacturing method thereof are applied to the field of dissolved oxygen concentration detection.