The invention discloses an OLED panel light emitting layer manufacturing method. The OLED panel comprises a substrate, an ITO anode, a hole injection layer, a hole transport layer, a light emitting layer, a cathode and a packaging layer, wherein the ITO anode, the hole injection layer and the hole transport layer are together called as an intermediate layer; and the light emitting layer needs to be manufactured above the intermediate layer. The light emitting layer manufacturing method comprises the following steps: the ITO anode, the hole injection layer and the hole transport layer are sequentially manufactured on the OLED substrate; an ink-jet printhead is then placed above the OLED substrate, and a light emitting material is injected to the ink-jet printhead; the ink-jet printhead and the OLED substrate are vertical, and the distance between an ink-jet hole in the ink-jet printhead and the OLED substrate is ensured to be 1 mm. Light emitting materials for three colors of RGB are made at one time, and the efficiency is improved; print on demand is adopted, and the material utilization rate is improved; and a manufacturing process is simplified, the OLED panel light emitting layer can be manufactured with cost reduced, and yield improvement of products is improved.