The invention discloses an intelligent operation and maintenance system for an IT computer room. The system comprises an acquisition terminal, an information analysis and processing system, and a mobile client, wherein the acquisition terminal is used for collecting environment characteristic values and hardware equipment information of the computer room, and running state information of operating systems and application software of equipment; the information analysis and processing system is used for storing data acquired by the acquisition terminal into a basic database, carrying out centralized and classified displays of the stored data, carrying out query analysis and exporting, advanced deployment and problem solving for the stored data, and storing the data acquired by the acquisition terminal; and the mobile client is used for manually carrying out data releases and queries through a mobile phone APP. The system adopting the technical scheme has the advantages that a computer software system can replace manual operations in continuous monitoring, customized analysis, abnormality warning, information releases, intelligent deployment and recovery and the like for the running of software, the equipment and an environment of an IT system, so that the IT operation and maintenance efficiency is greatly improved, and loads on the personnel are reduced.