The invention relates to an anti-shellfish coating for concrete surface protection of a water-conveying tunnel. The coating comprises a first component and a second component in a weight ratio of 10: 3 to 10: 5, wherein the first component comprises epoxy resin, a toughening modifier, a diluent, a thixotropic agent and a nanometer mixed filling material, and the second component comprises a benzyl alcohol diluent, the nanometer mixed filling material and at least one selected from a group consisting of a polyamide curing agent, a phenolic aldehyde amine curing agent and a modified aliphatic amine curing agent. The coating provided by the invention has the beneficial effects of improvement of the anti-carbonization, impervious performance and scouring and abrasion resistance of concrete used for the water-conveying tunnel, reduction in the roughness coefficient of a river and in shellfish erosion, prolongation of a repair cycle, enhancement of operation benefits and concrete life and substantial increase in economic benefits.