The invention belongs to the field of biotechnology and method, and particularly relates to a silicon column rapid regeneration method. The method comprises the steps that a silicon column is taken for adding distilled water to carry out centrifugal separation; silicon column regeneration liquid is added and for 3 minutes' standing to carry out centrifugal separation; the silicon column regeneration liquid is a phosphoric acid solution; the steps are repeated five times; after the silicon column is washed with L distilled water, then the centrifugal separation is conducted; residual moisture in the silicon column is removed completely, and then deionized water cleaning is conducted. The silicon column rapid regeneration method aims at the regeneration and utilization of the silicon column in the PCR cleaning kit or the agarose glue extraction kit, which can save the fund for the laboratory, save the resources for the society; the method is a more environment-friendly method in using the silicon column.