The invention discloses a light tube adhesive dispensing and curing system. The light tube adhesive dispensing and curing system comprises a light tube conveying line, a curing conveying line, a light tube transferring mechanism, a dispenser, slitting machines arranged on two sides of the dispenser and a light bar transferring mechanism for transferring light bars on the slitting machines to the dispenser; the slitting machines are used for slitting a soft substrate into the light bars; the light bar transferring mechanism transfers the light bars on one slitting machine and then transfers the light bars on the other slitting machine; the dispenser comprises fixing rods, a horizontal drive mechanism and dispensing heads arranged right above one ends, near one light tube, of the fixing rods; when the horizontal drive mechanism drives the fixing rods to move towards the direction of the light tube, the dispensing heads dispense an adhesive on light bars on the fixing rods; the light tubes corresponding to the fixing rods on the light tube conveying line are working light tubes; the horizontal drive mechanism drives the fixing rods to penetrate into the corresponding working light tubes; a lifting mechanism is arranged below the working light tubes; and a pressing mechanism is arranged above the working light tubes. Through the adoption of the light tube adhesive dispensing and curing system disclosed by the invention, the working efficiency is greatly improved.