A nucleic acid detection apparatus is disclosed. The apparatus includes a cap 1, a fixed liquid storage tank 2, test paper 3, standard sample tubes 4, a reaction reagent tube 5, puncturing nails 6 and liquid guiding paper 7. The apparatus can achieve one-time rapid detection after amplification of a plurality of nucleic acids. Operation is more convenient than operation of traditional nucleic acid amplification chromatography detection. The apparatus has good leakproofness and can effectively prevent environment pollution caused by amplification products. The apparatus reduces rigid requirements of present nucleic acid amplification detection methods on experiment sites, reduces operation difficulty and technical requirements on operators, and can achieve nucleic acid detection in the wild or in non-professional laboratories.