The invention relates to a pixel unit and a driving method, a display panel and a display apparatus, and aims to solve the problem demanding prompt solution in the aspect of enabling the display panel to satisfy requirements of high PPI and wide color gamut. The pixel unit comprises at least two sub pixels; each sub pixel comprises a first electrode, a first light-emitting layer, a second electrode, a second light-emitting layer and a third electrode which are arranged on a substrate in sequence, wherein the first light-emitting layer and the second light-emitting layer included in the at least one sub pixel are different in colors; and each light-emitting layer in at least two sub pixels at least comprises three different colors. According to the pixel unit, the number of colors of light rays emitted by the single sub pixel can be increased; in addition, each pixel unit at least comprises the light-emitting layer of three different colors, so that full color of each pixel unit can be realized by only using two sub pixels; and therefore, the number of the sub pixels in the pixel unit is reduced, and the display panel can satisfy requirements of high PPI and wide color gamut more easily.