The invention relates to a micro-fluidic chip device used for diagnosis of AIDS and based on a dual-driving coupling operation mode and belongs to the field of analytical testing. Cheap and easily-processed polydimethylsiloxane, namely PDMS is used for manufacturing a substrate of a micro-fluidic chip for diagnosis of AIDS. But there exist a series of problems. The scheme aims to solve the series of problems. According to main points of the scheme, PDMS with primitive surface is selected as the substrate, and a miniature ultrasonic transducer is fixedly installed at the position near a sample fluid terminal of the micro-fluidic chip. Meanwhile, an ultrasonic attenuator is arranged at a sampling end of the micro-fluidic chip so as to rapidly decrease ultrasonic intensity progressively in a short distance. Therefore, interfacial tension difference is formed between two ends of the chip. The different provides a force for driving the sample fluid to flow along a hydrophobic capillary channel towards the terminal. The force and a mechanical pumping force of a micropump contained in the structure are intercoupling with each other. Thus, the problems are solved.