The invention discloses a method for producing L-glufosinate-ammonium by using transaminase and an ethylene-forming enzyme. According to the method, 2-carbonyl-4-(hydroxyl methyl phosphoryl) butyric acid or salt thereof is taken as a raw material, and the L-glufosinate-ammonium is obtained through a catalytic reaction of the transaminase and the ethylene-forming enzyme under the condition of taking glutamic acid or salt thereof as an amidogen donor. According to the method, the 2-carbonyl-4-(hydroxyl methyl phosphoryl) butyric acid or the salt thereof is taken as a substrate, and the L-glufosinate-ammonium is obtained through the co-catalysis of the transaminase and the ethylene-forming enzyme under the condition of taking the glutamic acid or the salt thereof as the amidogen donor, so that by-product alpha-dibasic ketonic acid after a transamination reaction is completely converted into carbon dioxide and ethylene through catalysis of the ethylene-forming enzyme, under the condition of guaranteeing few using amount of the raw materials, the conversion rate of the raw materials is obviously improved, the cost of the raw materials is reduced, the subsequent purification technology is simplified, and the product total recovery is improved.