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发明授权
CN107151692B 一种鉴定猪活体背膘厚的方法及基因诊断试剂盒 有权

一种鉴定猪活体背膘厚的方法及基因诊断试剂盒
摘要：
本发明公开了一种鉴定猪活体背膘厚的基因诊断试剂盒。利用本发明的基因诊断试剂盒来鉴定猪活体背膘厚方法为如下(1)或(2)或(3)或(4)：(1)是检测猪个体的MEG3基因的第460位脱氧核糖核苷酸是T还是A还是T和A；(2)是检测猪个体的MEG3基因第二外显子的第1137位脱氧核糖核苷酸是A还是G还是A和G；(3)是检测猪个体的MEG3基因第二外显子的第1158位脱氧核糖核苷酸是A还是G还是A和G；(4)是检测猪个体的MEG3基因第一内含子的第51位脱氧核糖核苷酸是T还是C还是T和C。通过实验证明：本发明的方法与猪达100kg体重活体背膘厚的实际测定结果一致，对选择优良猪种有重要意义。
摘要（英）：

The present invention discloses a gene diagnosis kit for identifying the back-fat thickness of a living pig. The method for identifying the back-fat thickness of a living pig by using the gene diagnosis kit is the following method (1) or (2) or (3) or (4): (1) detecting whether the 460 site deoxyribonucleotide of the MEG3 gene of an individual pig is T or A or T and A; (2) detecting whether the 1137 site deoxyribonucleotide of the second exon of the MEG3 gene of an individual pig is A or G or A and G; (3) detecting whether the 1158 site deoxyribonucleotide of the second exon of the MEG3 gene of an individual pig is A or G or A and G; and (4) detecting whether the 51 site deoxyribonucleotide of the first intron of the MEG3 gene of an individual pig is T or C or T and C. According to the present invention, experimental results prove that the determination result of the method of the present invention is consistent with the actual determination result so as to provide important significance for the selection of excellent pig breeders.

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