The invention provides a synthesis method of a TS-1 titanium silicalite molecular sieve. The synthesis method comprises the following steps: directly physically mixing a silicon source, a titanium source and a templating agent without additionally introducing water and adding a seed crystal, aging at the low temperature of 60-80 DEG C, crystalizing at the temperature of 160-180 DEG C, finally washing, separating, drying and roasting to obtain a molecular sieve product, wherein the silicon source uses inorganic silicon (solid silica, silicic acid and silica sol) which is cheap and is easy to get; the templating agent uses tetrapropylammonium hydroxide (the use amount is obviously lower than that of the traditional method) and can also use the combination of at least one of tetrapropylammonium bromide and n-butylamine and sodium hydroxide. The disclosed synthesis method of the TS-1 titanium silicalite molecular sieve is simple, feasible, economical and environmentally friendly, and has an application value.