The invention belongs to the technical field of packaging materials, and particularly relates to a high-surface-energy long-carbon-chain polyamide transparent film, as well as a preparation method and an application thereof. The high-surface-energy long-carbon-chain polyamide transparent film is prepared from raw materials including long-carbon-chain polyamide material, wherein the long-carbon-chain polyamide is polyamide having the number of methylene between adjacent amide groups on the main chain being more than or equal to 10, and the surface tension of the high-surface-energy long-carbon-chain polyamide transparent film is 32-45mN/m; and the long-carbon-chain polyamide material is one or more of long-carbon-chain polyamide, additive-containing long-carbon-chain polyamide, long-carbon-chain polyamide alloy or long-carbon-chain polyamide nano-composite material. The high-surface-energy long-carbon-chain polyamide transparent film has the advantages of excellent mechanical performance, good size stability, good oil resistance and good gas barrier property, shows good printing performance under the condition that corona and other surface treatment means are not adopted, has excellent transparent property, and has a wide market prospect in packaging and printing technical fields.