The invention relates to an analysis method for determining stereoisomers and intermediates in solifenacin succinate by adopting high-performance liquid chromatography. The analysis method is characterized in that high-performance liquid chromatography (HPLC) is adopted, and isocratic elution is carried out on an amylose-tris(3,5-dimethylphenyl carbamate) bonded silica gel chiral HPLC column; a mobile phase is n-hexane-absolute ethyl alcohol-isopropyl alcohol-diethylamine solution (65:15:20:0.1) (volume ratio); and the mobile phase is adopted to dissolve a sample. The method can be used for simultaneously determining enantiomers and a diastereoisomer of the solifenacin succinate and intermediates as mutual enantiomers, thus providing a more reliable guarantee for the research on new synthesis process routes for the solifenacin succinate and quality control.