The present invention belongs to the technical field of biological prevention and control and specifically discloses a preparation method of a modified feed for aleuroglyphus ovatus and a breeding method for the aleuroglyphus ovatus. Wheat bran, corn bran, yeast powder, wheat germ powder, sucrose, water and potassium sorbate are mixed using a certain method to obtain the modified feed, and the modified feed is used to enable the aleuroglyphus ovatus to breed rapidly. The preparation method is simple in operations, can effectively improve yield of the aleuroglyphus ovatus, and has a positive effect on a large scale production of predatory mites belonging to amblyseius.