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发明公开
CN107177628A 一种利用农杆菌浸染法致使白花兜兰基因转变的方法 无效 - 驳回

一种利用农杆菌浸染法致使白花兜兰基因转变的方法
摘要：
本发明公开了一种利用农杆菌浸染法致使白花兜兰基因转变的方法，包括以下步骤：白花兜兰液体培养基的配制—白花兜兰固体培养基的配制—农杆菌液体浸染液的配制—白花兜兰浸染—白花兜兰暗培养—白花兜兰光照培养—结果检测。本发明利用白花兜兰最敏感的农杆菌LBA4404作浸染基质，成功实现了白花兜兰基因转变技术，第一次公开了白花兜兰基因转变的方法，并且本发明公开的白花兜兰转基因技术操作简便，结果具有可重复性，为白花兜兰的基因工程育种、遗传学、分子生物学研究提供了方便、快速、高效的理论基础和实验导入方法，为进一步通过转基因技术改造白花兜兰奠定了技术基础，值得推广。
摘要（英）：

The invention discloses a method for causing paphiopedilum emersonii gene conversion by utilizing an agrobacterium dip-dyeing process. The method comprises the following steps: preparing a paphiopedilum emersonii liquid medium, preparing a paphiopedilum emersonii solid medium, preparing an agrobacterium liquid dip-dyeing solution, die-dyeing paphiopedilum emersonii, darkly culturing paphiopedilum emersonii, illuminating the paphiopedilum emersonii and detecting a result. According to the invention, the most sensitive agrobacterium LBA4404 of paphiopedilum emersonii is used as a dip-dyeing medium, a paphiopedilum emersonii gene conversion technology is successfully realized, the method for paphiopedilum emersonii gene conversion is firstly disclosed, the paphiopedilum emersonii gene conversion technology disclosed by the invention is simple and convenient in operation, the result has repeatability, a convenient, quick and efficient theoretical basis and an experiment leading method are supplied for the genetic engineering breeding, genetic and molecular biological research on the paphiopedilum emersonii, a technical basis is established for further reforming the paphiopedilum emersonii through a transgenic technology and the method is worthy of popularization.

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