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发明公开
CN107177690A 蜜蜂幼虫腐臭病病原双重TaqMan荧光定量PCR检测方法 失效 - 权利终止

蜜蜂幼虫腐臭病病原双重TaqMan荧光定量PCR检测方法
摘要：
本发明属于病原检测技术领域，公开了一种蜜蜂幼虫腐臭病病原双重TaqMan荧光定量PCR检测方法，所述蜜蜂幼虫腐臭病病原双重TaqMan荧光定量PCR检测方法利用两条探针，一条探针5′端采用比较成熟的FAM作为荧光报告基团，另一条探针5′端选择VIC作为标记，两条探针3′端选用BHQ1非荧光淬灭基团。本发明的方法与其它病原无交叉反应；对P.larvae和M.pluton的检测灵敏度均达10拷贝/μL的阳性质粒；重复性试验结果显示批内、批间变异系数均小于2％。
摘要（英）：

The invention belongs to the technical field of pathogen detection and discloses a dual TaqMan fluorescent quantitative PCR detection method for foulbrood pathogens. According to the dual TaqMan fluorescent quantitative PCR detection method for the foulbrood pathogens, two probes are used, relatively mature FAM is adopted at the 5' end of one probe to serve as a fluorescent reporter group, VIC is selected at the 5' end of the other probe to serve as a marker, and BHQ1 non-fluorescent quenchers are selected at 3' ends of the two probes. The method has the advantages that cross reactions with other pathogens are avoided, the detection sensitivity for P.larvae (Paenibacillus larvae) and M.pluton (Melissococcus pluton) reaches 10 copy/mu L of positive plasmids; repetitive testing results show that a coefficient of intra-array variation and a coefficient of inter-assay variation are both smaller than 2%.

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