The invention relates to a molybdenum disulfide-carbon composite flower ball as well as a preparation method and application thereof. The preparation method of the molybdenum disulfide-carbon composite flower ball comprises the following steps: S1, dissolving ammonium thiomolybdate into glycol under a stirring condition to form a clear solution, and adding glucose into the clear solution under a stirring condition; S2, transferring the solution obtained in the step S1 into a reaction kettle, heating for 20-24 hours at 200-240 DEG C, cooling, washing precipitates, centrifuging, and drying so as to obtain a solid product for later use; and S3, performing thermal treatment on the solid product obtained in the step S2 for 2-4 hours at 450-700 DEG C in the presence of an inert atmosphere, thereby obtaining the molybdenum disulfide-carbon composite flower ball. The molybdenum disulfide-carbon composite flower ball provided by the invention has a three-dimensional grade structure and can be widely applied to fields such as super capacitors, lithium ion battery electrode materials or photoelectric catalysts.