The invention discloses an edible fungi culture box. The edible fungi culture box comprises a base, wherein a left sealing cover and a right sealing cover are symmetrically hinged onto the base, the inner wall of the left sealing cover is hinged to a first connecting plate, the inner wall of the right sealing cover is hinged to a second connecting plate, an installation plate is hinged between the first connecting plate and the second connecting plate, the installation plate is provided with a culture dish, the culture dish is provided with a rectangular transparent cover, a plurality of air holes are formed in the side wall of the rectangular transparent cover, the upper wall of the rectangular transparent cover is provided with a water leak hole, a temperature-humidity sensor and a PH detector are arranged in the rectangular transparent cover, a liquid medicine box and a spraying atomizing apparatus are arranged in the left sealing cover and the right sealing cover, the liquid medicine box is communicated with the spraying atomizing apparatus by virtue of a water pipe, and the side walls of the left sealing cover and the right sealing cover are respectively provided with an illuminating heat lamp. By virtue of a two-layer protection way, the normal growth of edible fungi of a culture medium can be ensured, other bacteria are reduced, and the culture quality of the edible fungi is improved.