The invention relates to a conductive silver paste electron microscopic detection sample pretreatment method. The conductive silver paste electron microscopic detection sample pretreatment method comprises the following steps of (1) mixing a conductive silver paste with a first organic solvent, performing primary ultrasonic treatment, and standing the solution for layering; (2) eliminating the supernatant liquid of the product layered in the step (1), adding in a second organic solvent for secondary ultrasonic treatment to obtain a sample for electron microscopic detection, wherein the applied volume of the second organic solvent is 1-4 times as much as that of the conductive silver paste. According to the conductive silver paste electron microscopic detection sample pretreatment method, the conductive silver paste is dispersed into the solvent through ultrasonic treatment, and after an organic layer is removed, the solid materials are again dispersed into the solvent to an appropriate contention to obtain the sample applicable to observation through an electron microscope; scientific and technical personnel of the field can observe the sample through analyzing equipment and accordingly can clearly observe silver particles and roughly analyze the varieties and the volume of addition of additives.