The invention relates to a winding main insulation temperature gradient and frequency response correlation test device. The device comprises an experimental box, a high voltage side direct current power supply module, a low voltage side direct current power supply module, a flow velocity control acquisition module, an experimental box, a temperature sensor, a temperature acquisition module, a dielectric spectrum tester and a computer. A high voltage winding, a low voltage winding and winding main insulation are located in the experimental box. According to the invention, through the output power of each section of the high voltage side direct current power supply module and the low voltage side direct current power supply module, the temperature gradient distribution of the winding main insulation of a transformer under actual load loss is simulated; the frequency response of the windings is measured by the dielectric spectrum tester; and through computer data processing, the correlation between the temperature gradient of the winding main insulation and the frequency response is explored, which helps to further understand the insulation state inside the transformer.