The invention provides a high-nutrition cell enlarged culture method. The method comprises the following steps: completely sucking a medium by a pipette, adding 3-5 ml of PBS to wash cells 2-3 times, adding 1-2 ml of 0.38% trypsin to a cell culture dish, carrying out digestion at 37 DEG C for 3-5 min, adding 2-3 ml of a fresh medium, and using the pipette to blow the bottom of the cell culture dish 40-50 times; transferring the obtained liquid to a cell centrifuging tube, centrifuging the liquid under a 1000-1500 g/min condition for 3-10 min, removing the obtained supernatant by the pipette, adding 2-3 ml of the fresh medium, and using the pipette to blow the cells up and down 40-50 times; and averagely adding the cells to 3-8 cell culture dishes, and carrying out enlarged culture. The method has the advantages of reasonability, easiness in realization, fast growth speed of the cells, good state of the cells, clear boundary, brightness in observation under a microscope, many division phases, good form and dispersiveness, and facilitation of biology and health related researches.