The invention provides an adjustment method of a high-water-cut-stage rhombic inverted nini-spot well pattern of a multi-layer low-permeability sandstone oil reservoir. The method comprises the following steps that firstly, the superior direction on a reservoir plane is judged; secondly, a high-water-cut oil well is judged in the plane, and a high-water-cut layer in multiple oil layers in the longitudinal direction; thirdly, according to the water driving oil effect of the oil layers, the development layer series are divided; fourthly, for the high-water-cut oil wells, the high-water-cut oil layers are plugged, and separated layer water injection is conducted for water injection walls; fifthly, in the mode that the high-water-cut oil wells are turned into the water injection wells in the fourth step, the structure of the rhombic inverted nini-spot well pattern is adjusted. By means of the adjustment method, the inefficient water circulation of the layers high in permeability and thickness can be reduced, the water driving oil effect and the reserves producing degree of the layers low in permeability and thickness can be improved, the oil well outputs are further improved, the oil recovery rate is increased, and the final water drive recovery efficiency and the economic benefits of oil reservoir exploitation are improved.