利索-全球专利检索

  1. 登录
  2. 注册
发明授权
CN107219363B 用于检测结核感染T细胞的抗原、试剂盒及应用 有权

用于检测结核感染T细胞的抗原、试剂盒及应用
摘要：
本发明公开了一种用于检测结核感染T细胞的抗原，该抗原的氨基酸序列如SEQ ID NO.1～SEQ ID NO.28所示，任取SEQ ID NO.1～SEQ ID NO.28中的八条多肽协同ESAT‑6和CFP‑10多肽及衍生物共同作用，特异性刺激结核分枝杆菌感染的T细胞能特异性的分泌IFN‑γ，增加检测的灵敏度。本发明提供了一种新的可用于结核感染T细胞的检测试剂盒，使用该试剂盒，直接对外周血进行抗原刺激，无需分离外周血单个核细胞，检测具有较高的灵敏度和特异性，且操作简便，检测成本较低，具有较高的临床应用价值。
摘要（英）：

The invention discloses an antigen for detecting tuberculosis infection T cells. The amino acid sequence of the antigen is shown as SEQ ID NO.1 to SEQ ID NO.28; any eight polypeptides in SEQ ID NO.1 to SEQ ID NO.28 are coordinated with ESAT-6 and CFP-10 polypeptides and derivatives to have a common effect to specifically stimulate mycobacterium tuberculosis infected T cells to specifically secrete IFN-gamma, and the detection sensitivity is increased. The invention provides a novel detection kit for the tuberculosis infection T cells; when the kit is used, peripheral blood is directly subjected to antigen stimulation and mononuclear cells of the peripheral blood do not need to be separated; the detection sensitivity and the detection specificity are relatively high; the novel detection kit is simple and convenient to operate, has relatively low detection cost and has relatively high clinical application value.

公开/授权文献
0/0