The invention provides a light-emitting diode quantum efficiency measurement device and a measurement method thereof, and relates to a light-emitting diode. The measurement device comprises a computer, a digital power meter, a temperature control source meter, a temperature control clamp, a spectrometer and an infrared thermal imager. The measurement method is characterized by adopting the light-emitting diode quantum efficiency measurement device; setting a group of temperature values T through the temperature control source meter, wherein the set group of temperature values at least comprise five different temperature values T; carrying out measurement according to the temperature values and current values to obtain a group of quantum efficiency eta of the light-emitting diode under different temperature control clamp temperature values TS; based on the obtained group of quantum efficiency eta, drawing a relation graph between the quantum efficiency eta and temperature T under the condition of different current values I; and obtaining quantum efficiency (eta') under the condition of eliminating thermal influence through a formula.