The invention discloses a mask plate module, a film layer and a manufacturing method of an organic electroluminescent display panel. By adopting a plurality of opened type mask plates instead of an existing opened mask plate, the film layer with a lonely island pattern in a display area is manufactured; opening areas of all opened type mask plates are not overlapped with each other, and all opening areas are spliced to form a pattern of the display area encircling the lonely island pattern, thereby ensuring that a shielding pattern requiring connection with an edge through a communication structure is not included in the opening areas of the all opened type mask plates. By sequentially adopting the all opened type mask plates as the shielding pattern, evaporation plating on the same mother plate by adopting the same material can be carried out, an evaporation plating film layer encircling the lonely island pattern can be formed in the display area, corresponding to all display substrates, in the mother plates, so that the pattern of the manufactured shielding film layer is not affected by the communication structure; therefore, the organic luminescent display panel comprising at least one lonely island pattern can be manufactured as demanded.