The invention provides a sports bottle with a seal structure, comprising a bottle body and a bottle cover; the bottle body is provided with an opening, and the bottle cover is threadedly connected to the opening of the bottle body; the sports bottle also comprises a seal ring, a clamp ring and a plurality of positioning mushroom steps, the bottom of the bottle cover is provided with a circular groove for receiving the seal ring, the seal ring is an O-shaped seal ring made of rubber, an end face of the seal ring is provided with a plurality of channels, the outer side of the seal ring is provided with a clamp groove, the clamp ring is a plastic clamp ring, the clamp ring is connected into the circular groove, the clamp ring is embedded in the clamp groove, and the positioning mushroom steps are embedded in the corresponding channels; as the clamp ring of the sports bottle is embedded in the clamp groove to fix the seal ring, it is voided that the seal ring comes off during use; as the positioning mushroom steps are embedded in the corresponding channels to further fix the seal ring, it is ensured that the seal ring is stably arranged in the bottle cover during use and never separates from the bottle cover.