The invention relates to mechanical type ultralow-temperature freezing equipment and an application method thereof. By adopting the mechanical type ultralow-temperature freezing equipment, a main control circuit is connected with a Stirling refrigerator through a temperature controller to be used for regulating a refrigeration temperature of the Stirling refrigerator according to different samples, so that the preservation temperatures of the collected samples are regulated to 150 DEG C below zero to the minimum. The application method of the mechanical type ultralow-temperature freezing equipment comprises the following steps: rapidly placing the collected samples in the mechanical type ultralow-temperature freezing equipment for preservation, and regulating different temperatures according to different samples to enable collected sample tissues to be rapidly frozen to the needed temperatures; directly inputting information obtained after detecting the samples into a computer, and establishing a corresponding large database at the same time, wherein each two-dimensional code in the database corresponds to a sample and sample information; and carrying out data analysis by the database according to a written program, wherein an analysis result displays a sample trend as well as an individual body condition and the possibility that a person falls ill in future corresponding to each sample.