The invention provides a novel cell freezing medium. The novel cell freezing medium comprises 5-15 parts of dimethyl sulfoxide, 2-5 parts of hydroxyethyl starch, 5-10 parts of chitosan, 2-5 parts of glutathione, 1-4 parts of vitamin C, 0.3-4 parts of dextranum, 0.5-2 parts of propylene glycol, 0.1-1 part of N-(phenylmethyl)-2-(4-pyrimidinylamino)-4-thiazolecarboxamide and 80-90 parts of a basal culture medium. The novel cell freezing medium is reasonable in components, rich in nutrition, can also significantly reduce cell damage and improve the survival rate of frozen cells while nutrition needed by dormant cells is ensured, and also maintains a very good state on an aspect of proliferation.