The invention discloses an automatic fruit grading and screening device which is used for facilitating pretreatments on fruits before study. The automatic fruit grading and screening device comprises a base, a shell, a splitting mechanism, and a material feeding mechanism; the shell and the base are arranged in an up-and-down way, with a support board arranged between; a fixed cylinder is arranged in an inner cavity of the shell; a first rotation ring, a second rotation ring, a third rotation ring, a fourth rotation ring, a fifth rotation ring and a sixth rotation ring are orderly arranged from top to bottom in the fixed cylinder in a rotating way; multiple air floats are respectively arranged in the first rotation ring, the second rotation ring, the third rotation ring, the fourth rotation ring, the fifth rotation ring and the sixth rotation ring; the fixed cylinder, the rotation rings and the air floats make up the splitting mechanism; the material feeding mechanism is arranged under the shell; the material feeding mechanism mainly comprises a pedestal fixed on the base, a material containing plate installed on the base, a driving block arranged in an inner cavity of the material containing plate, and a support block arranged between the driving block and an inner wall of the material containing plate. The automatic fruit grading and screening device is capable of effectively realizing splitting of fruit clusters.