The invention discloses a healthcare enzyme with an oxidation-resistant function. The healthcare enzyme is prepared from a fluid matrix and an additive, wherein the fluid matrix is prepared from water and a bacterial solution. The healthcare enzyme is characterized in that the additive is prepared from one or multiple of radix astragali seu hedysari, fructus hippophae, fructus lycii, radix glycyrrhizae, radix puerariae, green tea, radix et rhizoma ginseng, poria cocos, radix panacis quinquefolii, herba rhodiolae, rhizoma polygonati, radix morindae officinalis, rhizoma dioscoreae, fructus mori, fructus jujubae, arillus longan, pericarpium citri reticulatae, radix codonopsis, folium ginkgo, fructus crataegi, radix salviae miltiorrhizae, herba gynostemmatis pentaphylli and brown sugar; the bacterial solution contains one or several of bifidobacterium adolescentis, bifidobacterium animalis, bifidobacterium infantis, lactobacillus acidophilus, lactobacillus crispatus, lactobacillus delbrueckii subspecies lactis, lactobacillus gasseri, lactobacillus helveticus, lactobacillus reuteri, lactobacillus rhamnosus, lactobacillus salivarius, streptococcus thermophilus, lactococcus lactis subspecies lactis, leuconostoc mesenteroides subspecies mesenteroides and the like as well as cultures conventionally used for the production and the processing of food. The healthcare enzyme provided by the invention has the functions of resisting oxidation and relieving physical fatigue.