The invention relates to a structure of a Schiff base compound, a preparation method and partial characters. The compound looks like a yellow powdered crystal, with a melting point of 227.9 to 228.2 DEG C and a molecular formula of C23H19N3, and the chemical name is 2-((E)-((E)-(1-biphenyl-4-yl) ethylidense)-hydrazono) methyl)-1-H-indole. The structure is as follows: as shown in the specification. The preparation method of the compound is simple and has a relatively strong fluorescence emission peak within a range of 300 to 650 nm. Furthermore, the Schiff base has the IC50 (median inhibitory concentration) for lung cancer cells A549 of 30.0 [mu]M and for breast cancer cells 4T1 of 26.5 [mu]M, which show that the compound has a relatively good inhibition effect on the two cancer cells.