The invention discloses a production method of cooled fresh keeping beef. The production method of cooled fresh keeping beef comprises the following steps of performing slaughtering, performing bloodletting, performing peeling, removing internal organs, and after carcasses are qualified through quarantine, performing rinsing with water; and then precooling the rinsed carcasses, then selecting the carcasses of which the pH value is 5.6-5.8, performing deboning and segmentation, performing trimming, performing vacuum packaging, performing hot dipping, performing cold dipping, placing the carcasses after cold dipping into the environment of minus 2 to 2 DEG C, and performing storage. Compared with the prior art, the production method disclosed by the invention has the advantages that the obtained fresh keeping beef has better safety, better hygiene and longer preservation period.