The present invention particularly relates to an anti-gastric ulcer pharmaceutical composition, a preparation method and uses thereof, wherein the raw materials comprise, by weight, 50-59 parts of liquiritin, 170-185 parts of glycyrrhetinic acid, and 180-200 parts of saikoside C. According to the present invention, liquiritin, glycyrrhetinic acid and saikoside C are selected as the raw materials, various raw materials are mutually matched according to the specific ratio and co-act, and the significant anti-gastric ulcer effect is provided; and the gastric ulcer treatment effect of the pharmaceutical composition is superior to the treatment effects of any two raw materials of the three raw materials and the treatment effects of any one raw material of the three raw materials, and the pharmaceutical composition has the synergistic effect.