The invention belongs to the field of fermentation of microorganisms, and particularly provides environment-protection enzyme having deodorization and air purification effects and a preparation process thereof. The key of solving the problems of peculiar smell and bacteria is that by mutual coordination of metabolism of organic acid, secondary metabolic products and probiotics with activity, which are produced in an environment-protection enzyme fermentation process, organic pollution gas is absorbed and converted in common, so that the deodorization and air purification effects can be achieved. In the process, other secondary pollutants are not introduced, so that the preparation process has the characteristics of environment protection, ecological property, few investment and obvious effect.