The invention relates to 3D glass light-sensitive black ink composition and a preparation method thereof; the 3D glass light-sensitive black ink composition comprises acrylic resin, a thixotropic agent, epoxy resin and a black pigment and is characterized in that the acrylic resin is carboxyl-containing acrylic resin, a side chain of each polymer unit of a main chain of the carboxyl-containing acrylic resin has more than one carboxyl group, the carboxyl-containing acrylic resin has an acid value of 50-200 mgKOH/g, the carboxyl-containing acrylic resin has a weight-average molecular weight of 4000-8000, the thixotropic agent contains silicon dioxide or organic silicon, wherein silicon accounts for 20-46% by weight of the thixotropic agent, and the thixotropic agent has DBP (di-n-butyl phthalate) absorption value of 150-300 g/100 ml. coating film prepared with the 3D glass light-sensitive black ink composition has the advantages of high light-sensitivity, high precision, high shaking power, high adhesion and the like, has the excellent effects of boiling resistance, salt spray resistance, acid and alkali resistance, aging resistance and the like, and also has the properties of no wrinkling upon heating and no shedding at high temperature.