The invention relates to a feed additive for increasing jejunum transport protein expression quantity of weaned pigs and application thereof and belongs to the field of feed. The feed additive is prepared from carvacrol, glutamine, butyrin, edible acid, pepsin, curcumin, vitamin B2, montmorillonite and a carrier. The chosen ingredients of the feed additive are all safe and have no hazardous residue and can improve transport capacity of gastrointestinal tracts to amino acid and improve absorbing ability of stomach and small intestines to nutrient substances. When the feed additive is applied to weaned pig feed, poor absorbing ability to nutrient substances caused by weaning stress of the weaned pigs can be obviously improved, the jejunum transport protein expression quantity can be increased, a normal growth rate of piglets is maintained, a diarrhea rate of the weaned pigs is reduced, and culture benefit is improved.