The invention discloses a multifunctional tool box and a limiting fixing device. The tool box comprises a box body, universal wheels, an electric apparatus room, a side cover, a motor, a fixed plate, a bearing, a screw, a push-pull frame, a supporting base, a nut base, a vertical plate, storage boxes, an upper cover, a pin shaft, a movable cover, a supporting rod, a fixed storage box, a first guide groove, a first lengthened guide block, a first guide rail, a first movable box body, a second guide groove, a second lengthened guide block, a third guide rail, a second movable box body, a locking device, supporting wheels, a side plate, a fourth guide rail, a third guide groove, the limiting fixing device and a second pin shaft. The limiting fixing device specifically comprises a limiting block, an operation piece, a fixing shell, a second spring and a locking head. The multifunctional tool box is reasonable and simple in structure, low in production cost and convenient to install and is moved to site through the universal wheels, the motor is started to drive the screw to rotate, the supporting base is moved up, the vertical plate and the storage boxes are moved up, and small tools or spare and accessory parts are conveniently stored in the storage boxes in a classified mode and conveniently used by workers.