The invention provides a process for preparing moisture-absorbent stretch-proof fabric with silk. The process includes: removing impurities from silk sequentially, immersing the silk in an ammonia water solution, and performing degumming, washing and drying to obtain degummed silk fiber; immersing the degummed silk fiber in a lithium bromide water solution for hydrolyzing and concentrating to be saturated, and removing lithium bromide in the system to obtain a saturated silk protein solution; adding an emulgator-polyglycerin stearate to the saturated silk protein solution for stirring in temperature adjustment, and performing ultrasonic treatment to obtain a pretreated silk protein solution; adding the pretreated silk protein solution to a sodium hydroxide solution for even mixing, adding methoxypolyethylene glycols and epoxy chloropropane for stirring in temperature adjustment, adjusting the pH value of the system to be acidic, adding chitosan, stirring in temperature adjustment, solidifying spinning to be formed, and performing interweaving to obtain the moisture-absorbent stretch-proof fabric. The process is simple in method and low in cost, and the obtained modified silk fabric is stable in property, good in hydrophily and biodegradable.