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发明公开
CN107312090A 抗平颏海蛇毒素抗体及其制备方法和应用 审中-实审

抗平颏海蛇毒素抗体及其制备方法和应用
摘要：
本发明公开了一种抗平颏海蛇毒抗体，其是采用平颏海蛇毒素经灭活而成的平颏海蛇毒素抗原免疫马匹产生的IgG抗体。该抗平颏海蛇毒抗体的制备方法包括如下步骤：步骤一：抗原制备：称取平颏海蛇毒素，然后进行灭活制得所述的平颏海蛇毒素抗原；步骤二：免疫马匹：将步骤一制得的所述的平颏海蛇毒素抗原与马细胞因子的佐剂混合后免疫马匹，收集所述马匹的血浆；步骤三：提取抗体：从步骤二制得的血浆中提取马抗平颏海蛇毒免疫球蛋白F(ab’)2。此外，本发明还公开了所述的抗平颏海蛇毒素抗体在制备抗海蛇血清制剂中的应用。本发明能保护人和动物在平颏海蛇咬伤时免受平颏海蛇毒素中毒，所获抗体特异性高，反应灵敏，适于大规模推广应用。
摘要（英）：

The invention discloses a lapemis curtus toxin resistant anti-body. The lapemis curtus toxin resistant anti-body is an IgG antibody generated by immunizing a horse with a lapemis curtus toxin antigen obtained through inactivation of lapemis curtus toxin. A preparation method of the lapemis curtus toxin resistant anti-body comprises the following steps: step 1, weighing lapemis curtus toxin, and inactivating the weighed lapemis curtus toxin to prepare a lapemis curtus toxin antigen; step 2: immunizing the horse: mixing the lapemis curtus toxin antigen prepared in the step 1 with an adjuvant of a horse cytokine, then immunizing the horse, and collecting plasma of the horse; and step 3, extracting the anti-body: extracting lapemis curtus toxin resistant immune globulin F(ab')2 from the plasma prepared in step 2. Besides, the invention also discloses application of the lapemis curtus toxin resistant anti-body to preparation of a sea snake serum resistant preparation. The lapemis curtus toxin resistant anti-body can protect a person and an animal, bitten by a lapemis curtus, against lapemis curtus toxin, and is high in specificity, sensitive in reaction and suitable for large-scale popularization.

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