The invention discloses a culture method and detection method for rat fibrotic lung cells. The culture method and detection method for rat fibrotic lung cells comprise the following steps: preparing a culture solution, a perfusion fluid and a rinsing solution; then sampling rat type II alveolar epithelial cells and carrying out primary culture; stimulating the type II alveolar epithelial cells with amiodarone so as to obtain fibrotic lung cells; and detecting the fibrotic lung cells. The culture method provided by the invention can acquire a great number of cells in a short period of time and accurately reflect the condition of amiodarone-induced pulmonary fibrosis in vivo and is of great significance to deep research on the influence of regulation and mechanism of the phenotypic transformation of the type II alveolar epithelial cells to delaying, or even reversion, of amiodarone-induced pulmonary fibrosis, and provides a research object for further exploration of the pathogenesis of amiodarone-induced pulmonary fibrosis at cell and molecule levels.