The invention relates to a nondestructive picking device of Coriolus versicolor, comprising a rotary mounting seat, a base, a negative pressure generator and movable grippers; a collecting trough mounted at one end of the inside of the base, a pressurized gas generator is mounted at the other end of the inside of the base, a driving motor is mounted in the base between the pressurized gas generator and the collecting trough, the rotary mounting seat is mounted a the top end of the base right above the driving motor, a first fixed arm of force and a second fixed arm of force are hinged to the top end of the rotary mounting seat, one end of the first fixed arm of force far away from the rotary mounting seat is hinged to a first movable arm of force. The pressurized gas generator, the negative pressure generator, a first mounting disc, a main gas guide pipe, branch gas guide pipes and vacuum suckers, the negative pressure generator transmits compressed air around the vacuum suckers so that the ambient forms a negative pressure ambient, Coriolus versicolor is sucked and picked via the vacuum suckers, nondestructive picking of Coriolus versicolor is achieved, and picking is very convenient.